pAAV-G-CMV-GFP-Blank Amp

1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgccc taggcgttac ataacttacg gtaaatggcc cgcctggctg 181 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 241 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 301 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 361 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 421 ctacgtttag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt 481 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt 541 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg 601 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg 661 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagaaccgag 721 tttaaactcc ctatcagtga tagagatctc cctatcagtg atagagagct agcgatatct 781 gactcgagcg tcccagacta cgcttgagtt taaacacgcg tggtgtggaa agtccccagg 841 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 901 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 961 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 1021 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 1081 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggccgccacc atggtgagca 1141 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 1201 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 1261 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 1321 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 1381 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 1441 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 1501 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 1561 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 1621 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 1681 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca 1741 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 1801 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtga cttaaggggt 1861 ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc 1921 ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct 1981 ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct 2041 gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca 2101 ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg 2161 gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt 2221 tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc 2281 ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttcacgtgc 2341 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc 2401 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg 2461 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc 2521 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg 2581 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 2641 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 2701 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg 2761 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg 2821 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 2881 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt 2941 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt 3001 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc 3061 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg 3121 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca 3181 gaggttttca ccgtcatcac cgaaacgcgc gagccgggag ctgcatgtgt cagaggtttt 3241 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg 3301 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc 3361 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 3421 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 3481 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 3541 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 3601 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 3661 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc 3721 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 3781 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 3841 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 3901 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 3961 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa 4021 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 4081 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 4141 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 4201 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 4261 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 4321 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 4381 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 4441 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 4501 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 4561 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 4621 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga 4681 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 4741 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 4801 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 4861 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 4921 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 4981 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 5041 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 5101 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt

1 cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcgtcgggcgaccttt 60 GGACGTCCGTCGACGCGCGAGCGAGCGAGTGACTCCGGCGGGCCCGCAGCCCGCTGGAAA 61 ggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcact 120 CCAGCGGGCCGGAGTCACTCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGA 121 aggggttcctgcggccgcCCTAGGcgttacataacttacggtaaatggcccgcctggctg 180 TCCCCAAGGACGCCGGCGGGATCCGCAATGTATTGAATGCCATTTACCGGGCGGACCGAC 181 accgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcc 240 TGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTATCATTGCGG 241 aatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggc 300 TTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATTTGACGGGTGAACCG NdeI | 301 agtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatg 360 TCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAGTTACTGCCATTTAC 361 gcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacat 420 CGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGATGAACCGTCATGTA 421 ctacgtttagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgt 480 GATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCATGTAGTTACCCGCA 481 ggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagt 540 CCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCA CMV prom(591,672)>>> | 541 ttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattg 600 AACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAAC SacI | 601 acgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcgtttagtg 660 TGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGTCTCGAGCAAATCAC 661 aaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagaaccgag 720 TTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGGTATCTTCTTGGCTC BglII | tetO reg(728,767)>>> EcoRV | | | 721 tttaaactccctatcagtgatagagatctccctatcagtgatagagagctagcgatatct 780 AAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTCTCGATCGCTATAGA XhoI SV40 prom(822,1090)>>> | | 781 gactcgagcgtcccagactacgcttgagtttaaacacgcgtggtgtggaaagtccccagg 840 CTGAGCTCGCAGGGTCTGATGCGAACTCAAATTTGTGCGCACCACACCTTTCAGGGGTCC 841 ctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtgg 900 GAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACC 901 aaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagc 960 TTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCG SV40 origin(989,1066)>>> | 961 aaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgccca 1020 TTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGT ORF_1 rf(3)(1056,1850)>>> | ORF_3 rf(5)(1050,1865)<<< SfiI | | | 1021 ttctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggc 1080 AAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCG EGFP reporter(1131,1847)>>> | 1081 ctctgagctattccagaagtagtgaggaggcttttttggaggccGCCACCatggtgagca 1140 GAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGCGGTGGTACCACTCGT 1141 agggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaa 1200 TCCCGCTCCTCGACAAGTGGCCCCACCACGGGTAGGACCAGCTCGACCTGCCGCTGCATT 1201 acggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctga 1260 TGCCGGTGTTCAAGTCGCACAGGCCGCTCCCGCTCCCGCTACGGTGGATGCCGTTCGACT 1261 ccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgacca 1320 GGGACTTCAAGTAGACGTGGTGGCCGTTCGACGGGCACGGGACCGGGTGGGAGCACTGGT 1321 ccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgact 1380 GGGACTGGATGCCGCACGTCACGAAGTCGGCGATGGGGCTGGTGTACTTCGTCGTGCTGA 1381 tcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacg 1440 AGAAGTTCAGGCGGTACGGGCTTCCGATGCAGGTCCTCGCGTGGTAGAAGAAGTTCCTGC 1441 acggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgca 1500 TGCCGTTGATGTTCTGGGCGCGGCTCCACTTCAAGCTCCCGCTGTGGGACCACTTGGCGT 1501 tcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagt 1560 AGCTCGACTTCCCGTAGCTGAAGTTCCTCCTGCCGTTGTAGGACCCCGTGTTCGACCTCA 1561 acaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaagg 1620 TGTTGATGTTGTCGGTGTTGCAGATATAGTACCGGCTGTTCGTCTTCTTGCCGTAGTTCC 1621 tgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactacc 1680 ACTTGAAGTTCTAGGCGGTGTTGTAGCTCCTGCCGTCGCACGTCGAGCGGCTGGTGATGG 1681 agcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagca 1740 TCGTCTTGTGGGGGTAGCCGCTGCCGGGGCACGACGACGGGCTGTTGGTGATGGACTCGT 1741 cccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagt 1800 GGGTCAGGCGGGACTCGTTTCTGGGGTTGCTCTTCGCGCTAGTGTACCAGGACGACCTCA hGH_PA term(1857,2333)>>> | 1801 tcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtgaCTTAAGgggt 1860 AGCACTGGCGGCGGCCCTAGTGAGAGCCGTACCTGCTCGACATGTTCACTGAATTCCCCA 1861 ggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgc 1920 CCGTAGGGACACTGGGGAGGGGTCACGGAGAGGACCGGGACCTTCAACGGTGAGGTCACG 1921 ccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttct 1980 GGTGGTCGGAACAGGATTATTTTAATTCAACGTAGTAAAACAGACTGATCCACAGGAAGA 1981 ataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacct 2040 TATTATAATACCCCACCTCCCCCCACCATACCTCGTTCCCCGTTCAACCCTTCTGTTGGA 2041 gtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctca 2100 CATCCCGGACGCCCCAGATAACCCTTGGTTCGACCTCACGTCACCGTGTTAGAACCGAGT 2101 ctgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgg 2160 GACGTTAGAGGCGGAGGACCCAAGTTCGCTAAGAGGACGGAGTCGGAGGGCTCAACAACC 2161 gattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtt 2220 CTAAGGTCCGTACGTACTGGTCCGAGTCGATTAAAAACAAAAAAACCATCTCTGCCCCAA 2221 tcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggc 2280 AGTGGTATAACCGGTCCGACCAGAGGTTGAGGATTAGAGTCCACTAGATGGGTGGAACCG 2281 ctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttcacgtgc 2340 GAGGGTTTAACGACCCTAATGTCCGCACTTGGTGACGAGGGAAGGGACAGGAAGTGCACG 2341 ggaccgagcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctc 2400 CCTGGCTCGCCGGCGTCCTTGGGGATCACTACCTCAACCGGTGAGGGAGAGACGCGCGAG 2401 gctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcgg 2460 CGAGCGAGTGACTCCGGCCCGCTGGTTTCCAGCGGGCTGCGGGCCCGAAACGGGCCCGCC NarI | 2461 cctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctcc 2520 GGAGTCACTCGCTCGCTCGCGCGTCGACGGACGTCCCCGCGGACTACGCCATAAAAGAGG 2521 ttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctg 2580 AATGCGTAGACACGCCATAAAGTGTGGCGTATGCAGTTTCGTTGGTATCATGCGCGGGAC f1 origin(2588,2894)>>> | 2581 tagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgc 2640 ATCGCCGCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACG 2641 cagcgccttagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccgg 2700 GTCGCGGAATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCC 2701 ctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacg 2760 GAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGC 2761 gcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctg 2820 CGTGGAGCTGGGGTTTTTTGAACTAAACCCACTACCAAGTGCATCACCCGGTAGCGGGAC 2821 atagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgtt 2880 TATCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAA 2881 ccaaactggaacaacactcaactctatctcgggctattcttttgatttataagggatttt 2940 GGTTTGACCTTGTTGTGAGTTGAGATAGAGCCCGATAAGAAAACTAAATATTCCCTAAAA 2941 gccgatttcggtctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattt 3000 CGGCTAAAGCCAGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAA 3001 taacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgc 3060 ATTGTTTTATAATTGCAAATGTTAAAATACCACGTGAGAGTCATGTTAGACGAGACTACG 3061 cgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttg 3120 GCGTATCAATTCGGTCGGGGCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAAC 3121 tctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtca 3180 AGACGAGGGCCGTAGGCGAATGTCTGTTCGACACTGGCAGAGGCCCTCGACGTACACAGT 3181 gaggttttcaccgtcatcaccgaaacgcgcgagccgggagctgcatgtgtcagaggtttt 3240 CTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCTCGGCCCTCGACGTACACAGTCTCCAAAA 3241 caccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttatagg 3300 GTGGCAGTAGTGGCTTTGCGCGCTCTGCTTTCCCGGAGCACTATGCGGATAAAAATATCC 3301 ttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgc 3360 AATTACAGTACTATTATTACCAAAGAATCTGCAGTCCACCGTGAAAAGCCCCTTTACACG amp prom(3395,3423)>>> | 3361 gcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagac 3420 CGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTG amp marker(3465,4325)>>> | ORF_2 rf(3)(3465,4325)>>> | 3421 aataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatt 3480 TTATTGGGACTATTTACGAAGTTATTATAACTTTTTCCTTCTCATACTCATAAGTTGTAA 3481 tccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccag 3540 AGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTC 3541 aaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcg 3600 TTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGC 3601 aactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaa 3660 TTGACCTAGAGTTGTCGCCATTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTT 3661 tgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggc 3720 ACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCATAACTGCGGCCCG 3721 aagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccag 3780 TTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTC 3781 tcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataa 3840 AGTGTCTTTTCGTAGAATGCCTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATT 3841 ccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagc 3900 GGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCG 3901 taaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccgg 3960 ATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCC 3961 agctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaa 4020 TCGACTTACTTCGGTATGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTT 4021 caacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaa 4080 GTTGCAACGCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATT 4081 tagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctg 4140 ATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGAC 4141 gctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcag 4200 CGACCAAATAACGACTATTTAGACCTCGGCCACTCGCACCCAGAGCGCCATAGTAACGTC 4201 cactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcagg 4260 GTGACCCCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCC 4261 caactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcatt 4320 GTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAA 4321 ggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcattttt 4380 CCATTGACAGTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAA 4381 aatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaac 4440 TTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTG pBR322 origin(4480,5099)>>> | 4441 gtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgag 4500 CACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTC 4501 atcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcgg 4560 TAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCC 4561 tggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagca 4620 ACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGT 4621 gagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaaga 4680 CTCGCGTCTATGGTTTATGACAAGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCT AlwNI | 4681 actctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgcca 4740 TGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGT 4741 gtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgc 4800 CACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCG 4801 agcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctaca 4860 TCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGT 4861 ccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaa 4920 GGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTT 4921 aggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttc 4980 TCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAG 4981 cagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagc 5040 GTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCG 5041 gtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcgg 5100 CAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCC 5101 cctttttacggttcctggccttttgctggccttttgctcacatgt 5145 GGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGTGTACA